Potentiometric studies on calcium and magnesium binding by bullfrog skeletal muscle troponin C. Potentiometric titration curves.
نویسندگان
چکیده
منابع مشابه
A calorimetric study on calcium binding by troponin C from bullfrog skeletal muscle.
Microcalorimetric titrations of bullfrog (Rana catesbeiana) skeletal troponin C with Ca2+ were carried out in the absence of Mg2+ at 25 degrees C and at pH 7.0. The observed enthalpy titration curve was divided into three stages. The first stage of the titration (up to 2 mol of Ca2+/mol of protein) was characterized as an extremely exothermic process (delta H = -52 kJ/mol of site), the second o...
متن کاملPotentiometric titration and conformational change in pepsinogen.
Potentiometric titrations of pepsinogen as functions of ionic strength and temperature were studied. It was found that the ionic strength has a minor effect on the titration behavior of the protein. The maximum values of the buffering capacity in the pH range of 6.0 to 9.0 were found at pH values corresponding to those at which a conformational change of the protein occurs as determined by opti...
متن کاملARM - Based Automatic Potentiometric Titration System
Accurate measurement and analysis are very important tasks for many applications ranging from industrial operations, analytical laboratories to biological processes. In modern analytical chemistry, automatic titrators have more prominence than manual interpretations. Various instruments have been developed and proposed in the past to carry out potentiometric titrations. Also there are suppliers...
متن کاملZn(2+)-heparin interaction studied by potentiometric titration.
Measurement of the decrease in pH that accompanies the addition of Zn2+ to heparin in solution provided an indirect method of examining cation-polyanion interaction. Construction of plots analogous to isothermal saturation binding plots revealed the existence, for defined conditions of interaction, of a [heparin]-independent direct proportionality between the fraction of the maximal pH change o...
متن کاملPotentiometric Titration Curves of Aluminium Salt Solutions and Its Species Conversion in the Hydrolysis-polymerization Course
A new concept of critical point is expounded by analysing the potentiometric titration curves of aluminium salt solutions under the moderate slow rate of base injection. The critical point is defined as the characteristic spot of the Al salt solutions potentiometric titration curve, which is related to the experiment conditions. In addition, the changes of critical points reflect the influence ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1989
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.65.211